Publications of A.V. Greco

This page shows all publications that appeared in the IASI annual research reports. Authors currently affiliated with the Institute are always listed with the full name.

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IASI Research Report n. 294  (Previous    Next)


Mingrone G., Greco A.V., Bertuzzi A., Arcieri-Mastromattei E., Tacchino R.M., Marino F., Finotti E., Castagneto M.

Tissue uptake and oxidation of disodium sebacate in man.

ABSTRACT
In order to better ascertain its possible use as an alternative fuel substrate in total parenteral nutrition, sebacate (Sb) metabolism was studied in 7 overnight fasting healthy male volunteers, who received i.v. a constant infusion (99 mmoles over 8 hours) of disodium sebacate. Sb oxidation rate was determined using an isotopic sebacate (disodic salt of (1-10) C-sebacic acid) infusion (100 ?Ci from the 4-th to the 8-th hour of the cold sebacate infusion). Blood samples were collected during and after sebacate infusion at intervals of 30 min and Sb serum concentrations were determined by high performance liquid chromatography. Excreted radioactivity (?Ci/min) was measured by bubbling the expired air into an apparatus containing 3 mEq hyamine to trap CO2 from 20 liter Douglas-bag. CO2 production and O2 consuption were measured before and at 4 and 8 hours after starting the infusion. Twenty-four hours nitrogen exretion with urine was obtained. The RQ and the percent of calories derived from lipid oxidation were calculated by indirected calorimetry. The Sb serum level at the plateau phase was (mean ?SD) 4.54 ? 0.71 ?mole/ml, the overall rate of tissue uptake was 180.89 ? 4.50 ?mole/min, and the percent oxidation was 6.14 ? 0.44%. At the end of Sb infusion the RQ dropped to 0.839 ? 0.043, the percent of calories dues to sebacate oxidation was 1.59 ? 0.52%, and the calories derived from lipids increased to 37.77 ? 12.90%. These data show that a definite amount of the sebacate infused is oxidized in human tissues.
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