Publications of Alessandro Bertuzzi

This page shows all publications that appeared in the IASI annual research reports. Authors currently affiliated with the Institute are always listed with the full name.

You can browse through them using either the links of the following line or those associated with author names.

Show all publications of the year  1994, with author Bertuzzi A., in the category IASI Research Reports (or show them all):


IASI Research Report n. 379  (Next)


Bertuzzi A., Mingrone G., Gandolfi A., Greco A.V., Ringoir S., Vanholder R.

Binding of indole-3-acetic acid to human serum albumin

ABSTRACT
Indole-3-acetic acid, a product of tryptophan metabolism, is commonly considered to be a uremic toxin. Protein binding may affect the free concentration and hence the toxicity of this compound. In the present paper, the binding of indole-3-acetic acid to defatted human serum albumin at 37?C and pH 5, 7.4, and 8.5 was studied by equilibrium dialysis. The binding of indole-3-acetic acid in human serum was also investigated. Binding to defatted albumin was analyzed by a mathematical model assuming two independet high affinity binding sites plus a class of low affinity sites. For the site with highest affinity we found the following association constants: 6.80 x 10^3 M^-1 at pH 5, 8.96 x 10^3 M^-1 at pH 7.4, and 5.25 x 10^3 M^-1 at pH 8.5. In addition, a large number of low affinity sites was evidenced. The pH dependence of the highest affinity constant was investigated by a theoretical model. The experiments performed in serum suggested the presence of competitors which influence the binding of indole-3-acetic acid minly to the albumin sites with high affinity.
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