Publications of Alessandro Bertuzzi

This page shows all publications that appeared in the IASI annual research reports. Authors currently affiliated with the Institute are always listed with the full name.

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Show all publications of the year  1994, with author Bertuzzi A., in the category IASI Research Reports (or show them all):


IASI Research Report n. 388  (Previous    Next)


Bertuzzi A., Del Grosso N., Gandolfi A., Carmela Sinisgalli, Starace G.

Cell cycle analysis by the relative movement approach: effect of variability across S phase of DNA synthesis rate

ABSTRACT
Cell populations pulse labelled with BrdUrd and sampled at increasing times after the pulse yield DNA-BrdUrd distributions from which the relative movement (RM) and the depletion function (DF) of labelled undivided cells can be calculated. In this paper we present an extension of the equation for the time course of RM, given by White & Meistrich (Cytometry 7, 486-490, 1986), to the case in which the rate of DNA synthesis changes across S phase. Computer simulations showed that different patterns of DNA synthesis rate across S phase can result in appreciably different RM curves. An analytical expression of the RM curve, in which the variability across S phase of the rate of DNA synthesis is accounted for by only one parameter, was proposed. This expression was used for the simultaneous fitting of time sequences of RM and DF data of U937 cells, in order to estimate the transit times T_s and T........., and the population doubling time T_d. The use of the extended model gave better results than those obtained under the assumption of constant rate of DNA synthesis across S phase.
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