Analysis of Repeated Sequences in the Human Transcriptome: Experimental Challenges and Practical Examples The analysis of repeated sequences in the human transcriptome presents a significant challenge in genomic and transcriptomic research. These sequences, which include elements such as transposons and microsatellites, play crucial roles in gene regulation and genomic stability, yet their structural complexity makes accurate identification and characterization difficult. In this presentation, I will explore the main experimental challenges encountered in the analysis of repeated sequences, such as alignment difficulties. I will illustrate these issues with examples from recent scientific literature and my own experiments, highlighting the strategies employed to overcome these challenges. Additionally, we will discuss the biological implications of repeated sequences and their potential impact on human physiology and diseases